BIOL 2263e Human Anatomy & Physiology Lab II
Respiration
Lung Capacity Lab 23 points
Measuring Tidal Volume
A. (1 pt) IN YOUR OWN WORDS describe or define Tidal Volume.
Method
1. Prepare the spirometer according to Spirometer Instructions (found in Content).
Also read: Laboratory Procedures for Primate Osteometrics
2. Sit near spirometer setup, breathing quietly for 2 minutes
3. Place the tubing mouthpiece between lips, inhale in a normal, unforced way, pinch the nose closed with your fingers, and exhale in a normal, unforced way, into the tube.
4. Calculate the displaced water volume (original volume – remaining volume).
5. Record the displaced volume in the space to the right of Trial 1.
6. (3 pt) Refill the jug and Repeat twice more (for total of three readings).
Trial
Tidal Volume
1
2
3
Total
Expected/Normal Value
Average (total /3)
B. (1 pt) Briefly compare the subject’s Tidal Volume to expected / normal Tidal Volume
Measuring Expiratory Reserve Volume
A. (1 pt) IN YOUR OWN WORDS describe or define Expiratory Reserve Volume.
Method
1. Prepare the spirometer according to Spirometer Instructions.
2. Sit near spirometer setup, breathing quietly for 2 minutes.
3. Place the tubing between lips, plug the nose and take a normal breath or two through the mouth. After a normal exhalation, exhale all additional air possible into the tube.
4. Calculate the displaced water volume (original volume – remaining volume).
5. Record the displaced volume in the space to the right of Trial 1.
6. (3 pt) Refill jug the jug and repeat twice more (for total of three readings)
Trial
Expiratory Reserve Volume
1
2
3
Total
Expected/Normal Value
Average (total /3)
B. (1 pt) Briefly compare the subject’s Tidal Volume to expected / normal Expiratory Reserve volume
Measuring Inspiratory Reserve Volume
A. (1 pt) IN YOUR OWN WORDS describe or define Inspiratory Reserve Volume.
Method
1. Set up the spirometer for use.
2. Sit near spirometer setup, breathing quietly for 2 minutes.
3. Breathe in as deeply as possible, place tube between lips and exhale normally (without force)
4. Calculate the displaced water volume (original volume – remaining volume).
5. Subtract your AVERAGE tidal volume (from first Table) from the displaced volume to find the Inspiratory Reserve volume.
6. (3 pt) Record the calculated volume; refill the jug and repeat twice more (for total of three readings)
Trial
Inspiratory Reserve Volume
1
2
3
Total
Expected/Normal Value
Average (Total/3)
B. (1 pt) Briefly compare the subject’s Inspiratory Reserve Volume to expected / normal Inspiratory Reserve value
Measuring Vital Capacity
A. (1 pt) IN YOUR OWN WORDS describe or define Vital Capacity.
Method
1. Set up the spirometer for use.
2. Subject stands near spirometer breathing slowly and deeply for a while
3. Breathe in as deeply as possible
4. Place the tube between lips and breathe out as forcibly as possible
5. (6 pts) Calculate and record the displaced volume (original volume – remaining volume); repeat twice more.
Vital Capacity
Trial
Measured Vital Capacity
Calculated Vital Capacity*
1
2
3
Total
Expected/Normal Value
Average (total /3)
*Vital Capacity measurements can be checked by comparing to the sum of:
Tidal Volume + Expiratory Reserve Volume + Inspiratory Reserve Volume
B. (1 pt) Briefly compare the subject’s Vital Capacity to expected / normal Vital Capacity
